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What is CD11B+ and F4/80 negative population ResearchGate

The Prg4 gene-trap (Prg4GT) mouse (stock no. 025740, JAX, USA) is born lacking Prg4 expression which can be restored via CRE-mediated recombination [26]. The PrgGT/GT animal displays significant synovial hyperplasia and subintimal fibrosis and recombination appeared to attenuate these changes [23]. In … See more Joint capsular tissues were isolated, pooled from two knee joints to generate an independent sample, and digested using collagenase-D (2mg/mL) (Sigma Aldrich, USA) + 1μL RNase … See more IA injections were performed in the knee joints of 2-month-old mice under inhaled isoflurane gas anesthesia (5% induction and 2–3% maintenance). The mouse skin was shaved and … See more The bone marrows of the femurs and tibias of 2-month-old Prg4GT/GT and Prg4+/+ animals were isolated, and BMDMs were generated as described [36, 38]. On day 7, M2a BMDMs were induced by IL-4 + IL-13 (20 … See more Statistical analyses were conducted as we previously described [23]. We used unpaired, paired Student’s t test, and one-way or two-way ANOVAs followed by Tukey’s post hoc tests, with statistical significance set at … See more WebApr 3, 2024 · Here, approximately 35% of the CD11b + CD45 low (CD45 lo) cells showed F4/80 on the cell surface, while up to 70% of the CD45 hi cell population expressed both F4/80 and GR1 (Fig. 2f, g). Hence, microglia with CD45 lo expression seemed slightly stimulated showing induced expression of F4/80, whereas microglia/macrophages with … clayton homes and vanderbilt mortgage

【实用干货】如何用流式检测巨噬细胞 - 知乎 - 知乎专栏

WebWe identified unique, site-specific expression patterns of F4/80, CD80, CD86, CX3CR1, CCR2, FLT3, CD103, and MHC-II. Furthermore, we observed the two known CD45-positive populations (CD45 (high) and CD45 (int) ) in the brain, whereas liver, lung, and spleen exhibited a homogeneous CD45 (high) population. WebThe F4/80 molecule was established as a unique marker of murine macrophages when a monoclonal antibody was found to recognize an antigen exclusively expressed by these cells. However, recent research has shown that F4/80 is expressed by other immune cells and is not equivalently expressed across tissue-specific macrophage lineages, including ... WebApr 12, 2010 · Antibodies were purchased from eBioscience (San Diego, CA, USA; CD45, CD11c, F4/80, CD80, CD86 and Ly6G) and BD Bioscience (MHC II and AAD). Fresh kidney suspension was first incubated with anti-mouse CD45-FITC for 30 min on ice, and after washing, the cells were stained for 15 min on ice with fluorochrome-labelled monoclonal … down shake it off