WebBroad Ghost Peaks Contaminated inlet or pneumatics. Remove the column and bake out the inlet. Use a high-quality septum. Replace the split vent fi lter. Install an in-line fi lter between the pneumatics and the inlet. Incomplete elution of previous sample. Increase the fi nal oven program temperature or total run time.

Gas Chromatography-Mass Spectroscopy - University of …

WebThis document is believed to be accurate and up-to-date. However, Agilent Technologies, Inc. cannot assume responsibility for t he use of this material. WebThey are typically carryover from a previous run or ghost peaks from extra material being introduced in to the system. Eliminating cross-contamination, running long enough and hot enough, and keeping things clean will help … birds of prey silhouette identification

Troubleshooting Chromatographic Contamination Ghost peaks/ca…

WebApr 13, 2006 · Hi Simon From your description of the shape of the ghost peak, and assuming that you are using a non-polar column (please post the column description - there should be a label on the column with all the details), the ghost is a free fatty acid, and its disapperance after you do a blank run confirms that it is coming from the previous … WebThis can then result in “ghost peaks” in subsequent chromatographic analyses. Septa particles and sample residues can build up in split lines and cause a plug, which will result in problems maintaining proper flow to the inlet. Finally, on some GC systems, to access the inlet liner, a large nut to which the split line is attached must be ... WebFeb 28, 2024 · Peak Broadening 1. Injector: a. Sample carryover could cause the peaks to appear broader. To resolve these issues, look at the ghost peak injector solutions. 2. Column: a. The column film selected could be too thick, which would result in the peak to broaden. The retention of compounds could be reduced by decreasing the film thickness … danbury hospital nursing jobs